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American Society of Microbiology ABMM Sample Questions:

1. A centrifuge rotor containing capped tubes of sputum concentrate for AFB culture breaks during operation inside a Class II Biological Safety Cabinet (BSC). What is the most appropriate immediate action for the technologist?

A) Open the centrifuge within the BSC, discard broken glass in a regular sharps container, and clean the spill with 10% bleach.
B) Keep the BSC running, notify the supervisor, evacuate the immediate area, and post warning signs.
Allow aerosols to settle (e.g., 30 min) before initiating decontamination with a tuberculocidal disinfectant.
C) Carefully remove any intact tubes, wipe them with 70% alcohol, and place them in a new rotor to continue processing.
D) Immediately turn off the BSC, open the sash fully, and spray disinfectant widely.

2. A child presents with a characteristic "slapped cheek" rash followed by a lacy, reticular rash on the trunk and extremities. The causative agent is a single-stranded DNA virus belonging to the family Parvoviridae, known as:

A) Measles virus
B) Human parvovirus B19
C) Rubella virus
D) Roseola virus (HHV-6)

3. A clinical laboratory receives a cerebrospinal fluid (CSF) sample from a 65-year-old patient presenting with fever, headache, and nuchal rigidity. Gram stain reveals Gram-positive cocci in pairs and short chains. Culture on blood agar grows alpha-hemolytic colonies that are optochin-susceptible and bile soluble. Which organism is most likely identified?

A) Streptococcus pneumoniae
B) Staphylococcus aureus
C) Enterococcus faecalis
D) Streptococcus agalactiae

4. A patient with a history of neutropenia following chemotherapy develops a severe pneumonia.
Bronchoalveolar lavage fluid reveals the presence of hyaline, septate hyphae with frequent dichotomous branching at acute angles. Culture yields a filamentous fungus that produces conidiophores with a vesicle bearing phialides that release chains of conidia. The MOST likely genus is:

A) Aspergillus
B) Rhizopus
C) Fusarium
D) Mucor

5. A diagnostic laboratory is validating a new multiplex PCR panel for respiratory pathogens. According to CLIA regulations, which of the following parameters must be established by the laboratory performing the validation for this qualitative nucleic acid amplification test?

A) Accuracy, Precision, Analytical Sensitivity, Analytical Specificity (including cross-reactivity)
B) Accuracy, Precision, Reportable Range, Analytical Sensitivity
C) Accuracy, Precision, Clinical Sensitivity, Clinical Specificity
D) Precision, Reportable Range, Analytical Specificity, Reference Interval

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